Legend for all Panels

Panel 1. Profile of phosphosites in stationary phase (day one to day eight), exponential phase and treated with Rapamacyin (Rapa). The graph shows Z-scores at different conditions obtained from the average intensity of phosphopeptides normalized to the protein intensity (n=3 biologically independent experiments). The original data can be found in Kroschwald et al.

Panel 2. Effect of PKA inhibitor on phosphosites intensity in the following conditions: exponential growth, heat shock and eight days of stationary phase. Changes of phosphorylation abundance upon PKA inhitor treatment (plus) are shown as log2 fold change over the non treated condition (minus) for all three independent replicates (black circles) and for the average of the replicates (red diamond). The original data can be found in Kroschwald et al.

Panel 3. Position of the phosphosite upon PKA inhibition in the dataset in the following conditions: exponential growth, heat shock and eight days of stationary phase. The volcano plot shows the changes compared to the untreated condition and the significance (pvalue BH adjusted). The selected phosphosite is marked as a blue circle in the plot. The original data can be found in Kroschwald et al.

Panel 4. Localization of the phosphorylation site (yellow circle) on the protein primary sequence. The visualization of the protein domains is based on the data obtained from the Uniprot protein database (http://www.uniprot.org/, April 2023).

Panel 5. Summary table. The table shows all phosphosites identified for a selected protein. The cluster to which the phosphosite is grouped and the PKA effect are reported.